Bacterial microbiome variation across symbiotic states and clonal lines in a cnidarian model

Introduction Exaiptasia diaphana is a popular model organism for exploring the symbiotic relationship observed between cnidarians and their microsymbionts. While physiological roles of algal photosymbionts (Symbiodinaceae) are well studied, contributions bacterial communities less defined in this system. Methods We investigated microbial variation distinct parts body state across four genets held identical environmental conditions using 16s rRNA gene amplicon sequencing. Results found differentially abundant taxa part that highlight these bacteria may play holobiont heterotrophy nutrient cycling. Beta-diversity analysis revealed states consistent with previous studies; however, we did not observe presence previously reported core microbiota. also community differences clonal lines, despite years rearing conditions. Conclusion These findings suggest microbiome greatly influenced by host genetics unpredictable influences.